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Stained cells were analysed using Fortessa ( Myh11-CreERt2/Confetti ) or C6 (wild-type on an Aria-fusion flow cytometry-assisted cell sorter (BD Bioscience). with 10-fold cross-validation was performed on the training set using the caret R

Tier I – This level of training is required for all users of the NSU Flow Core, and is the only training needed by users with prior experience with the BD Fortessa X-20 The Flow Core has designated 3 training levels dependant on a potential users experience with Flow Cytometry and BD based cytometry systems/software. Tier I – This level of training is required for all users of the NSU Flow Core, and is the only training needed by users with prior experience with the BD Fortessa X-20 BD LSRFortessa™ X-20, BD FACSCelesta™ or BD FACSAria™ III. What you will receive • A printed training manual that includes the presentations and exercises • Sample preparation protocols and reagent data sheets • A 2 GB memory stick to save FCS files of all data generated during the training • A Certificate of Completion As a frequently referenced high-parameter cell analyzers, the BD LSRFortessa brand provides power, performance and consistency for your research. Designed to be affordable and expandable, BD LSRFortessa cell analyzers have the flexibility to support the growing needs of multi-colour flow cytometry assays. Learn more about BD LSRFortessa products I am aware that the Fortessa will take up more > of my time in training and optimising experiments, so I want to prevent > unnecessary things ending up in my workload.

Bd fortessa training

BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified Flow Core staff. GETTING STARTED 1. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then We have two cell sorters (BD FACS Arias) that are operated by facility staff. We have five cell analyzers (2 BD LSRII, 1 BD Fortessa,1 Luminex Muse and 1 iQue Screener PLUS) available 24/7 for trained users.

BD LSRII (L01/L02) 1.0 Purpose The purpose of this procedure is to provide a comprehensive and consistent approach to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers. 2.0 Scope and Application This SOP applies specifically to the BD LSRII (L01/L02) instruments in the

2. Disconnect the air line (green) and fluid line (Blue) from the sheath container.

1.844.8.BD.LIFE (844.823.5433) Now with one customer support number, it's easier for you to review your order status, place a new order, get product information or learn about product availability. BD …

These are a 2-day interactive orientation online course for those with no/minimal experience in flow cytometry and shall be useful to those working on BD FACSCalibur™, BD LSR Fortessa™, BD FACSVerse™, BD Accuri™ C6. and BD FACSCanto™ II flow cytometer platforms. The BD special order research program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Tailored to the special needs of research at the leading edge of biomedical discovery, the program offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements. BD FACSMelody™ Online Training Program* *additional content available in the e-learning library. Getting Started (03:53) System Startup (06:40) Design Experiment Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.

The FCF is equipped with one BD LSRFortessa™ (Diagnostic Purpose) and a BD LSRFortessa™ X-20 (Research Purpose). A review of the BD LSR Fortessa X-20- Flow Cytometer .
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Turn on the computer. Log in to Windows as the Operator. The password is “BDIS”. 1.3.

BD LSR Fortessa See Scheduling for information on scheduling time on this instrument.
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Training can be provided for analytical instruments (BD LSR Fortessa X-20 and 2x BD FACS Canto II).

3. Depressurize the sheath container by pulling up on the vent valve. 4.

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

BD Biosciences - Cell Analysis We are committed to the delivery of quality education in cell analysis using BD's state-of-the-art flow cytometry instrumentation, reagents and methodologies. BD Biosciences offers training courses which provide an optimal combination of theory and practical hands-on training, allowing customers to take full advantage of BD Biosciences products. BD Biosciences San Jose, CA 95131 Tel 877.232.8995 Fax 800.325.9637 ResearchApplications@bd.com BD Biosciences European Customer Support Tel 32.2.400.98.95 Our LSR-Fortessas are 4/5 laser, 18 colour machines. We use saline as a sheath fluid, which is in 20 litre ‘FACSFlow’ boxes under the benches behind the machine. The Fortessas run at the same speed with the same software as the CantoII, but has the fluidics system of the FACSCalbur. The Fortessas are the […] BD FACSCelesta ™ multicolor cell analyzer . The BD FACSCelesta multicolor cell analyzer helps rapidly and accurately analyze cells in a compact, affordable package.

The BD special order research program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Tailored to the special needs of research at the leading edge of biomedical discovery, the program offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements. BD Accuri™ C6 Plus Training Program . Self-Installation Tutorial (self-paced) Collect Tab (04:15) Analyze Tab (03:00) Statistics Tab (02:40) Batch analysis Tab (02:19) Unclogging the SIP (06:00) Preventative Maintenance (03:52) Archive. Our archive of e-learning courses can be used for basic learning.